A biomimic reconstituted high density lipoprotein (rHDL) based system, rHDL/Stearic-PEI/VEGF complexes, was fabricated\nas an advanced nanovector for delivering VEGF plasmid. Here, Stearic-PEI was utilized to effectively condense VEGF plasmid\nand to incorporate the plasmid into rHDL. The rHDL/Stearic-PEI/VEGF complexes with diameter under 100 nm and neutral\nsurface charge demonstrated enhanced stability under the presence of bovine serum albumin. Moreover, in vitro cytotoxicity\nand transfection assays on H9C2 cells further revealed their superiority, as they displayed lower cytotoxicity with much higher\ntransfection efficiencywhen compared to PEI 10K/VEGF and Lipos/Stearic-PEI/VEGF complexes. In addition, in vivo investigation\non ischemia/reperfusion rat model implied that rHDL/Stearic-PEI/VEGF complexes possessed high transgene capacity and strong\ntherapeutic activity. These findings indicated that rHDL/Stearic-PEI/VEGF complexes could be an ideal gene delivery system for\nenhanced VEGF gene therapy of myocardial ischemia, which might be a new promising strategy for effective myocardial ischemia\ntreatment.
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